The Autopsy

Autopsy authorization

 

The Medical Examiner is authorized by statute to perform an autopsy on any body within his/her jurisdiction, and therefore does not need further consent.

 

Medical autopsies may be performed in any case where authorization has been given by a member of one of the following classes of persons in the following order of priority:      (1) The surviving spouse or state registered domestic partner;      (2) Any child of the decedent who is eighteen years of age or older;      (3) One of the parents of the decedent;      (4) Any adult brother or sister of the decedent;      (5) A person who was guardian of the decedent at the time of death;      (6) Any other person or agency authorized or under an obligation to dispose of the    remains of the decedent.

 

CAP recommends keeping wet tissue from forensic autopsies for 1 year

- for hospital autopsies, CAP recommends keeping tissue for 3 months

 

 

Electrolyte levels in decomposition

 

Postmortem vitreous potassium levels has been attempted to help determine the time of death but methods have been found to be invalid

Anything that accelerates decomposition, increases the level of vitreous potassium

 

Decomposition = Increased K+, Decreased Na+, Cl-, and Glu

 

Therefore if Na, Cl, or Glucose levels are high they are more significant than low values

 

Link to detailed chart of vitreous findings in different conditions:

 

http://emedicine.medscape.com/article/1966150-overview#aw2aab6b4

 

Rigor Mortis

 

“Stiffening” of the body after death due to postmortem muscle contraction. Begins in first in smaller muscles (fingers, jaw) then progresses to the limbs

 

Due to depletion of ATP with resultant development of a stable complex of actin and myosin , thus preventing muscles from relaxing

 

Maximal onset between 6-12 hours postmortem

Rigor mortis comes on much more quickly if decedent was exerting themselves before death (eg. drownings)

Persists for 36-48 hours (less in hot weather; longer in cold weather)

 

 

Using Fly Eggs to Estimate Time of Death

 

Generalized fly life cycle. They vary depending on the species and the temperature.

 

Eggs

present in clumps of up to 300

laying to hatching takes 1 day

Larva - 1st – 3rd instar stages

Takes ~ 4 days

 

Pre-pupa

migrates away from the corpse seeking a suitable pupation site, (usually in soil)

pre-pupa to pupa takes 4 days

Pupa

undergoes transformation from larval body form adult fly

pupa to emergence takes 10 days

Adult fly

feeds on protein from body fluids

lays eggs on corpse

emergence to egg laying takes 2 days

 

~19 days to get adult fly, and on ~day 21 they lay more eggs

Autolysis

 

- the breakdown of one's self by one's self; the aseptic breakdown of tissue

- maceration: "pure" autolysis in stillborn fetus; bc no bugs are yet in baby's gut, little to no putrefaction occurs; looks like a burn, does not smell

- sepsis, insects, and high ambient temperature speed up tissue breakdown; where low temps slow it down

- determining cause of death in decomposed body can be difficult/impossible (must distinguish pre-/postmortem injuries, look for tool marks, do x-rays, toxicology, other lab tests)

 

Putrefaction - breakdown of tissue by bacteria, usually in large bowel

- almost always starts as a little green patch in right lower abd

- later can see linear red discolorations as bacteria invade BVs

-- "marbling" caused by breakdown of BVs, at which point not possible to get blood sample

 

Skeletonization

 

Mummification - decomposition in very dry envt

- embalming can also preserve tissue c chemicals

Adipocere

 

aka Grave Wax?

- fat undergoes hydrolysis to free fatty acids by virtue of endogenous lipases and bacterial enzymes

- bacterial enzymes, principally from Clostridium perfringens, convert these free fatty acids to hydroxy fatty acids.

- gray wax-like appearance; has waxy feel

This form of postmortem change usually requires high humidity or water submersion

 

 

Burns

 

At 120 F, exposure for 5 min can cause 3rd degree burns

- at 150 F, will get 3rd degree burns after 2 seconds